Journal: Biomedicines

Article Title: In Vivo Activity of Metal Complexes Containing 1,10-Phenanthroline and 3,6,9-Trioxaundecanedioate Ligands against Pseudomonas aeruginosa Infection in Galleria mellonella Larvae

doi: 10.3390/biomedicines10020222

Figure Lengend Snippet: Kaplan–Meier survival distributions for each P. aeruginosa strain (ATCC 27853, PAO1, CF1, CF2, and CF3) assessed over varying inoculum doses (3 × 10 0 to 3 × 10 5 CFU/mL). Significance was assessed through the log-rank (Mantel–Cox) test, and Holm’s correction was applied for multiple comparisons (*: p < 0.05, **: p < 0.01, ***: p < 0.001).

Article Snippet: Larvae infected with PAO1 (7.59 ± 0.71 × 10 6 /mL, p < 0.01), CF2 (8.61 ± 0.55 × 10 6 /mL, p < 0.05), and CF3 for 24 h (8.12 ± 1.06 × 10 6 /mL, p < 0.05) showed a significant increase in hemocyte density compared to the controls, ATCC 27853 (8.81 ± 0.57 × 10 6 /mL, p > 0.05) and CF1 (8.51 ± 0.39 × 10 6 /mL, p > 0.05) were not significant.

Techniques:

Journal: Biomedicines

Article Title: In Vivo Activity of Metal Complexes Containing 1,10-Phenanthroline and 3,6,9-Trioxaundecanedioate Ligands against Pseudomonas aeruginosa Infection in Galleria mellonella Larvae

doi: 10.3390/biomedicines10020222

Figure Lengend Snippet: Following the inoculation of G. mellonella with P. aeruginosa strains ATCC 27583 (3 × 10 4 CFU/mL), PAO1 (3 × 10 0 CFU/mL), CF1 (3 × 10 3 CFU/mL), CF2 (3 × 10 3 CFU/mL), and CF3 (3 × 10 0 CFU/mL), the ( A ) alteration in circulating hemocyte density and ( B ) bacterial burden, was assessed over a 24 h period. Data are presented as the mean ± SE of the three independent experiments. Statistical analysis was performed by comparing treatments to PBS injected controls at respective time points ( A ) and to prior time points ( B ) (*: p < 0.05, **: p < 0.01, ***: p < 0.001).

Article Snippet: Larvae infected with PAO1 (7.59 ± 0.71 × 10 6 /mL, p < 0.01), CF2 (8.61 ± 0.55 × 10 6 /mL, p < 0.05), and CF3 for 24 h (8.12 ± 1.06 × 10 6 /mL, p < 0.05) showed a significant increase in hemocyte density compared to the controls, ATCC 27853 (8.81 ± 0.57 × 10 6 /mL, p > 0.05) and CF1 (8.51 ± 0.39 × 10 6 /mL, p > 0.05) were not significant.

Techniques: Injection

Journal: Biomedicines

Article Title: In Vivo Activity of Metal Complexes Containing 1,10-Phenanthroline and 3,6,9-Trioxaundecanedioate Ligands against Pseudomonas aeruginosa Infection in Galleria mellonella Larvae

doi: 10.3390/biomedicines10020222

Figure Lengend Snippet: Survival (%) of G. mellonella inoculated with P. aeruginosa strains, ATCC 27853 ( A – D ), PAO1 ( E – H ), CF1 ( I – L ), CF2 ( M – P ), CF3 ( Q – T ), and treated with 2–10 µg/larvae of Mn-tdda-phen ( A , E , I , M , Q ), Cu-tdda-phen ( B , F , J , N , R ), Ag-tdda-phen ( C , G , K , O , S ), and gentamicin ( D , H , L , T ) over 96 h.

Article Snippet: Larvae infected with PAO1 (7.59 ± 0.71 × 10 6 /mL, p < 0.01), CF2 (8.61 ± 0.55 × 10 6 /mL, p < 0.05), and CF3 for 24 h (8.12 ± 1.06 × 10 6 /mL, p < 0.05) showed a significant increase in hemocyte density compared to the controls, ATCC 27853 (8.81 ± 0.57 × 10 6 /mL, p > 0.05) and CF1 (8.51 ± 0.39 × 10 6 /mL, p > 0.05) were not significant.

Techniques:

Journal: Biomedicines

Article Title: In Vivo Activity of Metal Complexes Containing 1,10-Phenanthroline and 3,6,9-Trioxaundecanedioate Ligands against Pseudomonas aeruginosa Infection in Galleria mellonella Larvae

doi: 10.3390/biomedicines10020222

Figure Lengend Snippet: Effect of treatment with Mn-tdda-phen, Cu-tdda-phen, and Ag-tdda-phen alone (1 µg/larvae) and in combination with gentamicin (CN) (1 µg/larvae) infected with ATCC 27853, PAO1, CF1, CF2, and CF3 on survival ( left ) and larval bacterial burden ( right ).

Article Snippet: Larvae infected with PAO1 (7.59 ± 0.71 × 10 6 /mL, p < 0.01), CF2 (8.61 ± 0.55 × 10 6 /mL, p < 0.05), and CF3 for 24 h (8.12 ± 1.06 × 10 6 /mL, p < 0.05) showed a significant increase in hemocyte density compared to the controls, ATCC 27853 (8.81 ± 0.57 × 10 6 /mL, p > 0.05) and CF1 (8.51 ± 0.39 × 10 6 /mL, p > 0.05) were not significant.

Techniques: Infection